Thursday, October 27, 2016

With one in three people expected to get some kind of cancer in their lifetime, heeding my advice could mean the difference between life and death. Here are the five signs you're likely to be diagnosed with cancer in your lifetime . . . Your body, and in particular your immune system, cannot function at optimal levels if . . . You are deficient or imbalanced in key nutrients and hormones. You are overweight or obese. You smoke or chew tobacco. You eat too many refined sugars and grains. You're ingesting toxins in your food, water, air, and medicines. OK, I know that sounds a little broad, but I'm going to be more specific as we go through my seven smart steps to prevent cancer.

5 Signs You Will Get Cancer And 7 Smart Ways to Prevent It! "You have cancer" is something you NEVER want to hear from your doctor . . . because it means a world of suffering, and, for too many, death. But here's SHOCKING news — most people have cancer in their bodies right now and don't even know it! That's because a healthy body and a strong immune system destroy emerging cancer cells before they can create the life-threatening disease . . . Dr. David Brownstein But some people are not so lucky. Sadly, over 5,000 people in North America are diagnosed with cancer every single day — almost 2 million per year! And that's why we're thrilled at Newsmax Health today to bring you an extraordinary presentation — How to Prevent Cancer — by America's foremost holistic health practitioner, Dr. David Brownstein. In the next few moments, Dr. Brownstein will reveal five warning signs that you are at risk for cancer. You'll be surprised at just how common these are. But the good news is that you DO NOT have to be a cancer victim. Today, Dr. Brownstein will show you what causes cancer, along with his simple steps to prevent and even reverse it. Prevention is more important than ever before because, as Dr. Brownstein will tell you . . . Contrary to cancer industry propaganda, we are NOT winning the war on cancer. Conventional cancer treatments are failing. And overall death rates for cancer are unchanged in 80 years! Dr. Brownstein is the medical director of the Holistic Center for Medicine in West Bloomfield, Mich. He's the author of 10 best-selling health books, and he's the nationally known editor of Newsmax's very popular Natural Way to Health. Let's go to Dr. David Brownstein now. His valuable guidance on preventing cancer can literally save your life, and the lives of those you love! How to Prevent Cancer By Dr. David Brownstein Dr. David Brownstein Hello, I'm Dr. David Brownstein. Today, I'm going to show you five warning signs that you're likely to get cancer, and also how to prevent it! And what I have to say will be of equal value to those who already have been diagnosed with cancer, are undergoing treatment, or are in remission. What you discover in the next few minutes can literally save your life . . . My goal is to empower you to take charge of your health, and not become a cancer victim because of a lack of this basic cancer-prevention knowledge. Why listen to me on this subject? I'm a U.S.-trained medical doctor with a private practice in West Bloomfield, Mich. I have had tremendous success restoring the health of patients who have come to me after conventional medicine has failed them. And what's particularly interesting about that is . . . Routinely, I find my new patients have key nutrient deficiencies and hormonal imbalances, usually as a result of eating the Standard American Diet. I refer to it as the SAD diet. This diet can predispose you to many nutrient and hormonal imbalances and leads to a devitalized body. I find they have been exposed to an overload of environmental toxins in food, water, air, and consumer products, and these exposures are suppressing and disrupting their immune systems. What's more . . . Their bodies have been damaged by harmful and unnecessary drugs. And finally, they've been misinformed by the government, the media, and most egregiously, by a medical establishment controlled by the pharmaceutical industry. The human body is a wonderful machine, but all of its millions of cells must function together harmoniously, and not be inhibited, harmed, or thrown into chaos. If they are, it sets the stage for chronic illnesses such as cancer to develop. You see, in order to significantly lower your risk of getting cancer, it is important to have your immune system function at optimal levels. That's what I'm going to show you today. How to Prevent Cancer And don't worry about taking notes because, just ahead, I'll show you a special offer to get a FREE copy of my report, How to Prevent Cancer, which includes everything we're discussing today and much more. Let's begin with some facts the "Cancer Industrial Medical Complex" doesn't want you to know . . . In North America, it is projected that one in three people will be diagnosed with cancer in their lifetime. In fact, we expect to see 1,825,400 new cases of cancer this year, and, sadly, a large percentage of these people will die from it. Each year, I receive the annual report from CA: A Cancer Journal for Clinicians, a summary of cancer statistics in the United States dating back to 1930. The most shocking chart shows the annual age-adjusted cancer death rates among men and women, and the sad truth is . . . Cancer death rates are mostly unchanged in 80 years! Surprised? Most people are. All the drug industry propaganda claims that we're making real progress in the war against cancer. But look at the facts . . . For males, since 1930, the only bright spot has been the decline in deaths for stomach cancer. The death rate for colon cancer is unchanged . . . for prostate cancer it's increased . . . and for lung cancer it's significantly increased. For women, while the death rates for colon, uterine, and stomach cancer have declined slightly, the rates are essentially unchanged for breast cancer, and they've dramatically increased for lung and ovarian cancers. For both men and women, the rates for thyroid cancer are rising at epidemic rates. Also consider that, right now, nearly one in seven women is expected to get breast cancer and one in three men is expected to get prostate cancer. And, as I previously stated, worst of all . . . One in three among the entire population is expected to get some kind of cancer during his or her lifetime. That's a frightening report with bad odds. Meanwhile . . . Conventional medicine would have you believe that chemotherapy, radiation, and surgery are effective treatments for cancer. But nothing could be further from the truth. The "Cancer Industrial Medical Complex" has wasted 80 years and an untold fortune on research aimed at killing cancer once it has been detected. But once cancer is detected, it is often too late for many patients. Meanwhile, almost no money has been spent on research for cancer prevention, or even searching for the underlying causes of cancer. And I can assure you doctors are not taught anything about this in medical school. Just ahead, I'll show you How to Prevent Cancer, which is now more important than ever before, considering conventional cancer treatments are failing and the death rates continue unabated . . . There is one aspect of the "cancer treatment story" that is an unqualified success — and that's the cancer industry's billion-dollar profits. Keep in mind that chemo drugs can cost $10,000, $20,000, even $30,000 a month. The treatment costs for just the top six cancers have topped $56 billion annually, and total cancer care now exceeds $110 billion annually. With billions of dollars in annual profits, do you think the industry will admit its failings and change course? And are they really looking for a cancer cure, or just more expensive — and very profitable — drugs for treatment? Regardless of how you view that, a very serious problem with cancer drugs has come to light . . . A recent study at the Fred Hutchinson Cancer Research Center in Seattle startled its researchers when . . . They discovered that chemotherapy causes healthy cells to "secrete a protein that sustains cancer tumor growth" and creates resistance to further treatment. This explains why chemotherapy initially appears successful, only to prove ineffective when the cancer returns, often with a vengeance. Likewise, surgery and radiation might remove or kill some cancer cells, but it's impossible to get them all, and the remaining cells are left to multiply and spread. Let me be blunt about this . . . Cancer is not caused by a deficiency of chemotherapy. There are underlying causes that are NOT being addressed by conventional medicine. And with billion-dollar profits, cancer treatment — not a cancer cure — is the priority for the drug industry. And that's why in the next few minutes, and in even greater detail in my special report, How to Prevent Cancer, I'm going to show you . . . How to avoid the things that are known to cause cancer. How to make it difficult for cancer to get started and grow. How to help your body naturally kill cancer cells. How to Prevent Cancer And the first thing I must stress is that cancer can only be prevented and cured by YOUR OWN HEALTHY BODY with an optimally functioning immune system. Although we're not sure exactly why, we know the cancer process: An abnormal group of cells fail to complete their normal life cycle and die on schedule. Instead, they live on, multiply and spread. And this is very important to understand — a healthy cell has a predetermined life cycle. It divides several times and then dies. Cell death is called apoptosis. Healthy cells eventually experience apoptosis and are replaced by new cells. That's normal. Cancer is an abnormal cell that keeps dividing, skipping the necessary step of apoptosis. And as it multiplies, it forms a tumor, and then parts of it break off and spread and metastasize throughout the body. Cancer patients die by starvation when there is too much cancer in the body and it consumes all of the body's fuel. Now, here's something critically important to know . . . Cancer cells are emerging in most people's bodies at various times. It can be accurately said that "everybody has cancer" at one time or another. Yes, right now you may have cancer cells in your body and not even know it! The only difference between a healthy person who never gets a cancer diagnosis and a sick person who does . . . is that the former has a healthy body and strong immune system, while the latter does not. Do you know how strong your immune system is? Is it strong enough to ward off cancer? With this in mind, the goal for any cancer prevention or treatment program is to avoid those things known to cause cancer, and to create the healthiest possible body with an optimally functioning immune system. And that's exactly what I'm going to show you today. With one in three people expected to get some kind of cancer in their lifetime, heeding my advice could mean the difference between life and death. Here are the five signs you're likely to be diagnosed with cancer in your lifetime . . . Your body, and in particular your immune system, cannot function at optimal levels if . . . You are deficient or imbalanced in key nutrients and hormones. You are overweight or obese. You smoke or chew tobacco. You eat too many refined sugars and grains. You're ingesting toxins in your food, water, air, and medicines. OK, I know that sounds a little broad, but I'm going to be more specific as we go through my seven smart steps to prevent cancer. And as we proceed, remember you don't have to take notes, because I'm going to give you a special offer to get a FREE copy of my fully detailed report, How to Prevent Cancer, which includes everything we're discussing today and much more. I also have some additional and very valuable cancer-prevention reports for you that discuss key areas in more detail. And these, too, are FREE with the special offer, but more on that in a moment. But . . . Let's first see how to prevent cancer . . . #1: Don't Smoke Smoking is the #1 cause of death by lung cancer among both men and women. Smoking also contributes to many other health problems, including heart disease, heart attacks and strokes, cataracts, premature births, low birth weight, hip fractures, numerous lung ailments, and throat and stomach cancer. Cigarette smoke contains carcinogenic chemicals, including arsenic, benzene, cadmium, polonium-210, vinyl chloride, formaldehyde, and other toxins. If you currently smoke, quit now. And if you know you'll have a hard time, see your doctor for medical help. The evidence is clear: If you're smoking, you're literally asking to get cancer. And the survival rate for lung cancer is very low. #2: Maintain Adequate Iodine Levels I'm sure this next one, which falls under the category of "nutrient deficiencies and hormonal imbalances," is a complete unknown to you, but it's critically important . . . We have an epidemic of "iodine deficiency" in this country, and I believe it's a major contributor to the increasing rates of cancer of the thyroid, breast, and prostate. Iodine is essential to every cell in your body, but it's especially important to your thyroid gland, which makes the hormone that regulates your entire body's metabolism. Iodine is also essential for healthy breast and prostate tissues, which require more iodine than other body tissues except your thyroid gland. Now consider this: Very early in the 20th century, we saw an epidemic of goiter in this country. In simple terms, goiter is a swollen thyroid gland. It can cause difficulty swallowing. And worse, it results in reduced thyroid hormone, a slower metabolism, weight gain, insomnia, fatigue, brain fog, depression, and, if left unchecked, can become cancerous. Obesity, which is just 20% over your ideal body weight, also puts you at greater risk for cancer. And this is just one result of a goiter-hampered thyroid gland. If your ideal slim weight is 163 pounds, you're obese and at risk for cancer if you weigh 195 pounds. In a five-year study almost 90 years ago, an Ohio physician, Dr. David Marine, was able to prove that the epidemic of goiter was caused by an iodine deficiency. And after he demonstrated that goiter could be cured with iodine, it was added to salt, and for many years, to bread. Go to your local supermarket and you'll see the dark blue canister of iodized salt. In fact, it's probably in your cupboard. But my research has shown that the amount of iodine in iodized salt is insufficient. What's more, in the 1970s, the baking industry replaced the iodine in bread with a cheaper substitute, bromine. And, today, the vast majority of breads, pastas, and baked goods are made with brominated flour. In a long list of dumb moves, replacing iodine with bromine was possibly the dumbest move ever made by food manufacturers because it has caused the epidemic of iodine deficiency that we are currently experiencing. The problem is that bromine binds to iodine receptors in your body, which inhibits the absorption of iodine, and that's a recipe for a health disaster. Already our agricultural soils are deficient in iodine, so the crops they produce are iodine deficient. Making matters worse, the FDA has set the RDA for iodine at least 100 times too low, which means you can't get enough iodine in your diet, or from iodized salt or a multivitamin. This iodine deficiency epidemic we are experiencing is causing serious problems with our thyroid and prostate glands and also is leading to diseased breast tissue. This is why we have a 30-fold increase in fibrocystic breast disease (lumpy tissue), which is often a precursor to breast cancer. And bromine is now ubiquitous in our modern world: It's used in medicines, as a disinfectant for swimming pools and spas, and in hundreds of consumer products, including the interior parts of automobiles. Buy a new car today and you'll immediately be exposed to a large amount of bromine. Sadly, conventionally trained doctors are completely unaware of this deficiency, even as more and more people develop thyroid, breast, and prostate cancer. And remember, once a patient is referred to an oncologist and they enter the "Cancer Industrial Medical Complex," they will receive ineffective treatment programs which rely on: Surgery Radiation Chemotherapy No conventional doctor will prescribe iodine, unless it's radioactive iodine designed to kill a thyroid tumor, and that is a harmful treatment. The simple and effective solution is supplementing with inorganic, non-radioactive iodine, which costs just a few pennies a day. How does inorganic, non-radioactive iodine prevent cancer? It promotes apoptosis, or cell death. And when it comes to cancer, apoptosis is the difference between good health and a potentially fatal cancer diagnosis. Now, are you ready for the SHOCKER? I always test for iodine levels, and I've found more than 95% of my patients are deficient. I suspect the same deficiency exists throughout the entire population, and it's a major contributor to cancer. For this reason, I want you to have a FREE copy of my special report: In these reports, you'll discover why iodine is so important, and what types of iodine and dosages I recommend to my patients to promote good health and cancer-cell apoptosis. And here's good news . . . Both of these reports are yours FREE today, just for trying a no-risk subscription to my monthly health advisory: . . . at less than 15 cents a day. Yes, you heard me right: Less than 15 cents a day! And each month in Natural Way to Health . . . I offer safe and effective natural alternatives to harmful drugs. I show you how to avoid — and cleanse your body of — disease-causing toxins in our foods and environment. I provide guidance on nutrition, supplements, and bioidentical hormones to achieve good health and an optimally functioning immune system. Plus . . . I sort out medical misinformation in the media and alert you to the false propaganda promoted by the drug industry. And answer readers' questions on major health topics. I'll tell you more about this special offer in a moment — because it also includes some additional valuable reports — but let's first get back to cancer prevention. #3: Avoid Synthetic Chemicals and GMOs in Your Food This one seems obvious. Nobody would ask for synthetic chemicals in their food, and yet hundreds of millions of people are getting them without even knowing it. That's because conventionally raised animals are fed diets that contain synthetic hormones, antibiotics to speed growth, and genetically modified organisms (GMO) in food sources that have been shown to cause cancer. And the food products that reach your table still have these dangerous chemicals in them. They're in dairy products, beef, chicken, pork, and farm-raised fish. On the other hand, organically grown animals are free of these chemicals, such as beef from cattle that graze on natural grass. Many people mistakenly think that eating red meat can cause heart disease and cancer, but the problem isn't the red meat, but rather the chemicals in it. If you buy organically grown beef, free of hormones and antibiotics, it's a healthy source of protein, as long as it's part of a balanced diet that also includes plenty of fresh fruits and vegetables. There's another problem with our common diet . . . The vast majority of conventionally grown fruits and vegetables sold in grocery stores are sprayed with pesticides and chemical fertilizers. And while that ensures a bigger crop for growers, it means more cancer, since these chemicals are known carcinogens. Here again, the solution is simple: Eat certified organic fruits and vegetables that are free of pesticides and chemical fertilizers. There's more to know about the foods you eat . . . Harmful, cancer-producing chemicals are found in all of the name-brand artificial sweeteners, including aspartame. One popular brand contains chlorine atoms. Chlorine is in the same family as bromine, and it will inhibit iodine absorption. As you've just learned, you need iodine, not only for overall good health, but for cancer-cell apoptosis. Eat products made with these chemicals, and your immune system may have trouble killing cancer cells. What's more, two of the biggest artificial sweeteners, ones that have been around for decades, have been shown to create cancer in laboratory animals. Aspartame, in particular, is suspected of causing brain cancer. And it's the most common artificial sweetener in diet sodas, sugar-free ice creams, and hundreds of diet products that claim reduced calories. Ironically, these chemically sweetened diet foods do very little to help people lose weight, and when you trade reduced calories for cancer, you've made a very bad bargain. What's the solution here? Instead of using artificial sweeteners, or products made with them, use pure organic stevia (without any additives), organic coconut crystals, or natural, raw honey. These will not harm your body. Keep in mind that raw honey should not be given to children 1 year of age and younger. OK, but how do you put all this together? It might seem intimidating and require that you make big changes in your diet. But not really . . . Just ahead, I'll show you a special subscription offer to Natural Way to Health. Let's learn more about preventing cancer . . . #4: Avoid Refined Sugars, Salts, Flours, and Oils Refined foods are foods that have been processed during manufacturing. Typically, the nutrients have been stripped out, while harmful sugars, oils, grains, and salts have been left in, or put in. This is done for cost efficiency and for longer shelf life, but these foods are essentially "devitalized." If you eat these devitalized foods, over time you'll get a devitalized body. And then your immune system won't work as it should. And that's exactly how you allow cancer to get a foothold. Remember, I always test my new patients for vitamins, minerals, and hormones, and I have found the vast majority to have deficiencies and imbalances. Obviously, this is a common problem with the American diet, so let me give you some examples of specific foods to avoid . . . Avoid refined white table sugar, as cancer loves refined sugar. It's literally the fuel cancer needs to grow. What's more, refined sugar has been shown to suppress the immune system for up to five hours. Think about that . . . If you snack on candy or soft drinks every few hours, and then finish your dinner with cake, pie, or ice cream, you are literally suppressing your immune system near continuously, and that's an open-door invitation to cancer and other chronic illnesses. When people are diagnosed with cancer, they are always shocked, but they never consider that too much sugar in their diet might be the culprit. Likewise, their oncologists never say, "Stop eating sugar immediately." Instead, they say, "You will have surgery, and then chemotherapy and/or radiation. Don't wait for that to happen: Eliminate refined sugars from your diet now! The natural sugars in fresh fruits are fine, but not the sugar added to bottled fruit juices, canned fruit, and fruit-flavored yogurts. If you like yogurt, eat plain organic yogurt and add your own fresh fruit, and if you need to sweeten it, add a little stevia or raw honey. Next, avoid refined flours, oils, and salts, all of which are unhealthy and put you at greater risk for cancer, as well as for arthritis, diabetes, and heart disease. Refined flours, grains and carbohydrate foods include: white bread white pasta white rice white potatoes cookies cakes chips almost all processed foods in boxes, bags, and cans All of these foods convert to sugar in your body, and again, cancer loves sugar. Sugar-coated cereals are bad for you and your children. Even unsweetened cereals made with refined grains are unhealthy. Again, these are refined carbohydrates that convert to sugar in your body. How much sugar are you consuming without even knowing it? Let me give you a few good choices right now — you can eat oatmeal, nuts, and all products made with organic whole grain flours that have not been bromated. You can eat yams, which are orange on the inside, but not white potatoes. You can eat all the fresh fruits and vegetables you want, but choose organic produce, as these will not have harmful pesticides and chemical fertilizers in them. Next, it's very important to avoid refined and processed oils. Bad fats and oils include: Margarine Canola Corn Cottonseed Soybean oils Any product whose label includes the words "partially hydrogenated" All of these have been shown to suppress the immune system over time by producing toxic trans fatty acids. They also lead to weight gain and heart disease. Healthy oils include: Organic butter Coconut oil Cold-pressed extra virgin olive oil Organic butter is a healthy fat that will not hurt you. Coconut oil is one of nature's "perfect foods" with great nutritional and healing powers. And olive oil has long been part of the healthy Mediterranean diet. Next, avoid common white table salt, which has been refined. It has been processed and bleached and is devoid of all nutrients. Instead, use organic, unrefined sea salt, which is natural and contains nutritious minerals. And unlike bleached table salt, unrefined sea salt tastes better and is a healthy food, even for people with high blood pressure. I recommend one half to one teaspoon daily for almost everyone. What's more, eating organic sea salt daily will also help you achieve an ideal anti-cancer body pH of 7.4. Now, all of this might sound a little difficult, but it's not. You're simply replacing bad foods with good foods, and you'll make the choices you want. 5 Common Foods That Damage Your Health You'll find that the good foods are more delicious, give you more energy, don't cause digestive problems, and will naturally reduce your weight. And speaking of bad foods, one more cancer-prevention FREE report I want you to have is: 5 Common Foods That Damage Your Health. In this report, you'll discover the very real dangers of soybean foods, artificial sweeteners, refined carbohydrate foods, and two other common foods that put you at a higher risk for cancer (they're probably in your refrigerator right now). Soybean foods have been promoted as healthy, but that's flat-out wrong. There is only one form of soy that won't do harm, and that's fermented soy. This is found in miso and tempeh. Every other non-fermented soybean food, including edamame and soy milk, is unhealthy and harmful to your body. Why have we been misled on this? Because soybeans are among the most profitable crops in the United States. They are extremely cheap to grow and food manufacturers use soy as a filler in many processed foods. Approximately 50% of all packaged foods in the grocery store contain some form of non-fermented soy that is harmful to you. You can thank Washington food manufacturing lobbyists for enshrining this misinformation in the FDA food pyramid. And that's one of the most important reasons to read my Natural Way to Health each month — I will tell you when the government, the food industry, and the drug industry are misleading you. Now, you may scroll down to the special offers to receive Natural Way to Health, and FREE copies of my reports — How to Prevent Cancer, Iodine: The Most Misunderstood Nutrient, 5 Common Foods That Damage Your Health . . . PLUS . . . One more report I want you to have — Bioidentical Hormones Can Save Your Life. To achieve your best health with an optimally functioning immune system, you must have sufficient and balanced hormones. I'll tell you what you need to know on the subject, how to get tested, and the cancer risks of synthetic hormones. Now, let's get back to our seven smart steps for cancer prevention . . . #5. Avoid and Remove Toxic Metals From Your Body In addition to testing my patients for iodine, vitamin, and mineral deficiencies, and imbalanced hormones, I also test for heavy metals, which include: Mercury Lead Cadmium Arsenic Nickel All of these heavy metals will suppress your immune system, and a compromised immune system cannot protect you from cancer. Over 20 years, I have tested tens of thousands of my patients for these heavy metals and have found that fully 70% have toxic levels — with mercury, one of the most poisonous substances known to man, being the biggest problem. Most of my patients don't have a clue where they picked up these harmful metals, but I can tell you they are in dental materials as well as the air, water, medicines, and food sprayed with pesticides and chemical fertilizers. Do you have high levels of these toxic metals in your body that are, right now, suppressing your immune system and putting you at risk for cancer? You'd better find out, before you're shocked with a cancer diagnosis! Mercury, the worst of all, is prevalent in: Albacore tuna Swordfish Shark Mackerel Shellfish It's also used in vaccinations, as well as in silver amalgam dental fillings. If you eat these types of fish, get annual vaccinations, or have had silver amalgam dental fillings at any time during your life, you are almost certain to have elevated levels of mercury in your system. When you take advantage of my special offer, you'll learn how to get tested for toxic metals. But here are some obvious signs . . . A suppressed immune system will cause: Fatigue More frequent colds and flus Allergies Rashes Infections Worse, it can cause: Autoimmune disorders Liver damage Parkinson's Alzheimer's Cancer Unfortunately, conventional doctors will NOT test you for heavy metals. And once these metals are in your body, they can only be removed by chelation therapy or chelating nutritional supplements. In my own practice, I have seen dramatic health improvements in patients who have had these heavy metals removed. How to Stop Environmental Toxins From Poisoning You Not only do my patients get sick far less often, but they have a lot more energy and feel years younger. There is some truth to the old saying, "Get the lead out!" For more details, see my special report, How to Prevent Cancer. But I also want you to have a FREE copy of my report, How to Stop Environmental Toxins From Poisoning You. In this report, I'll show you how to avoid toxic metals in the first place, how to get tested for them, and how to get them out of your body. By the way, if you don't have access to a holistic doctor who can provide IV chelation therapy to remove heavy metals . . . . . . you can do this with specific supplements. I'll tell you which ones to use, and the dosages I recommend to my patients. Remember, 70% of my patients have tested high for toxic levels of heavy metals. If you're serious about getting well, staying well, and preventing cancer, you must get these heavy metals out of your body. Of course, these are the kinds of valuable things I discuss in Natural Way to Health each month. In fact, before we return to cancer prevention, let me take just half a minute to tell you about some recent past issues — out of 54 total in the archives — all of which you can access as a new subscriber. Stop Heart Disease in Its Tracks, Naturally. Surprise! Cholesterol does not cause heart disease and nobody should be taking cholesterol-lowering statin drugs. See the simple natural solution to prevent heart disease that was proven 60 years ago but was suppressed by the drug industry. Statin drugs won't stop heart disease, but they will harm your heart, your muscles, your sex life, and also cause fatigue and brain fog. How to Use Iodine to Fight Breast Cancer. I recently had a patient diagnosed with severe fibrocystic breast disease, which is a precursor to breast cancer. Her oncologist recommended mastectomy. She saw me for a second opinion, and I prescribed a high daily dose of iodine. In six weeks, her fibrocystic breast disease was gone and her breasts were saved. Her oncologist was astounded, but had no interest in learning about iodine supplementation. The Essential Guide to Good Prostate Health. With half of all men expected to get BPH, a swollen prostate gland that makes urination difficult and painful, as well as one in three men projected to get prostate cancer during his lifetime, every man needs this guide to a healthy prostate. Make no mistake, conventional medicine has no good solutions here; your best course is the natural way. The Secrets to Fighting Arthritis. Virtually all of the arthritis drugs are ineffective and harmful. Some forms of arthritis are caused by a bacterial infection that can be treated with antibiotics. All others are caused by refined sugars and inflammation-causing ingredients in processed foods. See the smart, drug-free way to beat arthritis! The Natural Way to Beat Depression. Big Pharma propaganda would have you believe that depression is caused by a chemical imbalance that can be corrected with antidepressant drugs. FALSE! The correct way to beat depression is to clean up your diet of toxic foods, balance your hormones, get exercise, and take specific supplements that naturally bring about a happy state of mind. These are just a few of the 54 past issues in our archives that you can access, when you try a risk-free subscription to Natural Way to Health for less than 15 cents a day . All you have to do is scroll to the bottom of the page to take advantage of our special offers that start your subscription and give you FREE copies of my cancer-prevention reports. Now, let's finish up with the last two prevention steps . . . #6. Avoid Radiation Exposure All medical radiological tests use ionizing radiation. And ionizing radiation is a known carcinogen that can damage cell DNA and lead to abnormal cells that turn into cancer . These include traditional X-rays, dental X-rays, and mammograms. But the worst of the bunch are the PET (positron emission technology) scan, and the CT (computerized tomography) scan. PET and CT scans expose the body to much larger amounts of radiation. CT scans, in particular, produce 50 to 100 times the radiation of a traditional chest X-ray. There has been a dramatic increase in the number of CT scans ordered in the last decade — over 60 million of them — and the New England Journal of Medicine says at least one-third are unnecessary. I suspect two-thirds are unnecessary. Remember, the radiation from these tests is cumulative. The damage builds up over time. Consider this . . . A recent mega-study on mammograms concluded that 3,243 women, ages 40 to 59, would have to be tested annually for 10 years, just to save two lives. Yes, just two lives, for 10 years of testing on thousands of women that adds cancer risk. That's because . . . Ten years' worth of annual mammograms produces a cumulative effect of 10 rads of absorbed radiation, or about the same amount Japanese women received just one mile from the atomic bomb sites during WWII. That correlates to a 10% increased risk of breast cancer! That's not something you want, especially when there is a much better alternative . . . When you try a no-risk subscription to Natural Way to Health, you can access two very important issues in our archives: In these important issues, you'll discover what's wrong with conventional cancer screening tests and get safer and more accurate alternatives. In particular, I'll show you a much better screening test for breast cancer that my wife uses. Remember, you may scroll to the bottom of the page and you'll see our special offers to start your no-risk subscription to Natural Way to Health, including . . . Monthly issues of Natural Way to Health How to Prevent Cancer report, plus several BONUS reports Unlimited FREE access to our 54-issue archives And all of this is yours for less than 15 cents a day! But first, let's take a quick look at the seventh smart step for cancer prevention. #7. Test for Vitamin and Mineral Deficiencies By now, you know I test all of my patients for several things that conventional doctors DO NOT test for, and there's a reason for that. Because our medical schools have long been controlled by the pharmaceutical industry, conventional medical doctors are taught to diagnose pathologies and to prescribe specific drugs to treat those ailments. There is no emphasis on prevention or wellness, and no attempt at cures. It's all about administering drugs for treatment of illness and disease, and that's because that's where the profits are. But in recent times, some doctors have seen the failures of conventional drug-based medicine and have decided to take an entirely different approach . . . These are the holistic doctors who put the emphasis on prevention and wellness, as well as on natural therapies to restore good health. This idea is not revolutionary but simple common sense. Avoid those things that are known to cause harm, and give your body what it needs for optimal health. Let me show you what I've learned after testing thousands of my patients . . . These are the top 10 vitamin and mineral deficiencies I've found in the majority of my patients. I'm certain these deficiencies extend to the larger North American population . . . Iodine Vitamin B12 Magnesium Vitamin C Vitamin B1 (thiamine) And also . . . Vitamin D Sulfur Zinc Chromium Potassium You already know about iodine deficiency and how it can lead to cancer. But take the second item, vitamin B12, for example. Because of the typical American diet that creates heartburn and acid reflux, there is a huge business in antacids, including both over-the-counter antacids and prescription proton-pump inhibitors, which claim "just one pill a day." But these antacid drugs inhibit the absorption of vitamin B12, which is essential to digestion, energy production, mental function, and the proper functioning of your immune system. With tens of millions of North Americans taking antacids daily, they are walking around with a vitamin B12 deficiency that's compromising their immune systems, robbing them of energy and making them more vulnerable to cancer. Do you take antacids daily? When you read Natural Way to Health, you'll find effective natural solutions that make them completely unnecessary. You should know that conventional doctors do not study nutrition in medical school, and therefore don't have a clue what the optimal levels of vitamin and minerals should be. And then, the government's RDA for some of the most important nutrients is set 5, 10, 20, and even 100 times too low. You simply cannot achieve good health, stay healthy, and avoid disease, if you have nutrient deficiencies. For these reasons, you'll need to find a holistic doctor to run the tests we've been discussing today. And if you don't have a holistic doctor nearby, you can discuss with your doctor what you learn in Natural Way to Health each month. Remember, in addition to receiving Natural Way to Health each month, you'll get my full Cancer Prevention Kit, with our no-risk offers that run less than 15 cents a day. These offers include unlimited access to our 54-issue archive, covering virtually every important health topic. Plus, FREE copies of the special reports . . . 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Friday, October 21, 2016

Induced pluripotent stem cells (iPSCs) constitute a potential source of autologous patient-specific cardiomyocytes for cardiac repair,

ARTICLE PREVIEW view full access options NATURE | LETTER Print Share/bookmark 日本語要約 Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts Yuji Shiba, Toshihito Gomibuchi, Tatsuichiro Seto, Yuko Wada, Hajime Ichimura, Yuki Tanaka, Tatsuki Ogasawara, Kenji Okada, Naoko Shiba, Kengo Sakamoto, Daisuke Ido, Takashi Shiina, Masamichi Ohkura, Junichi Nakai, Narumi Uno, Yasuhiro Kazuki, Mitsuo Oshimura, Itsunari Minami & Uichi Ikeda AffiliationsContributionsCorresponding author Nature 538, 388–391 (20 October 2016) doi:10.1038/nature19815 Received 07 October 2015 Accepted 31 August 2016 Published online 10 October 2016 Article tools Citation Reprints Rights & permissions Article metrics Induced pluripotent stem cells (iPSCs) constitute a potential source of autologous patient-specific cardiomyocytes for cardiac repair, providing a major benefit over other sources of cells in terms of immune rejection. However, autologous transplantation has substantial challenges related to manufacturing and regulation. Although major histocompatibility complex (MHC)-matched allogeneic transplantation is a promising alternative strategy1, few immunological studies have been carried out with iPSCs. Here we describe an allogeneic transplantation model established using the cynomolgus monkey (Macaca fascicularis), the MHC structure of which is identical to that of humans. Fibroblast-derived iPSCs were generated from a MHC haplotype (HT4) homozygous animal and subsequently differentiated into cardiomyocytes (iPSC-CMs). Five HT4 heterozygous monkeys were subjected to myocardial infarction followed by direct intra-myocardial injection of iPSC-CMs. The grafted cardiomyocytes survived for 12 weeks with no evidence of immune rejection in monkeys treated with clinically relevant doses of methylprednisolone and tacrolimus, and showed electrical coupling with host cardiomyocytes as assessed by use of the fluorescent calcium indicator G-CaMP7.09. Additionally, transplantation of the iPSC-CMs improved cardiac contractile function at 4 and 12 weeks after transplantation; however, the incidence of ventricular tachycardia was transiently, but significantly, increased when compared to vehicle-treated controls. Collectively, our data demonstrate that allogeneic iPSC-CM transplantation is sufficient to regenerate the infarcted non-human primate heart; however, further research to control post-transplant arrhythmias is necessary. Subject terms: Induced pluripotent stem cells Stem-cell research At a glance Figures First | 1-3 of 14 | Last left Transplanted iPSC-CMs partially remuscularize infarcted cynomolgus monkey hearts. Figure 1 iPSC-CMs electrically couple with the host heart. Figure 2 Transplantation of iPSC-CMs improves cardiac contractile function. Figure 3 Electrical consequences of transplantation of iPSC-CMs. Figure 4 Characteristics of the HT4 haplotype. Extended Data Fig. 1 Generation of iPSCs from a MHC homologous cynomolgus monkey. Extended Data Fig. 2 Characteristics of G-CaMP7.09. Extended Data Fig. 3 Generation and purification of cynomolgus iPS cell-derived cardiomyocytes. Extended Data Fig. 4 Study protocol and design. Extended Data Fig. 5 Immune response following transplantation of iPS cell-derived cardiomyocytes. Extended Data Fig. 6 Macroscopic and microscopic analysis of iPSC-CM recipients. Extended Data Fig. 7 Summary of histological, mechanical and electrophysiological consequences. Extended Data Fig. 8 Additional electrical analysis of hearts transplanted with iPSC-CMs. Extended Data Fig. 9 Time course of left ventricular size and BNP levels. Extended Data Fig. 10 right Videos READ THE FULL ARTICLE Subscribe to Nature for full access: $199 Subscribe ReadCube Access*: $3.99 rent $9.99 buy *printing and sharing restrictions applyBuy/Rent now Purchase article full text and PDF: $32 Buy now Already a subscriber? Log in now or Register for online access. Additional access options: Use a document delivery service Login via OpenAthens Purchase a site license Institutional access References References• Author information• Extended data figures and tables• Supplementary information Deleidi, M., Hargus, G., Hallett, P., Osborn, T. & Isacson, O. Development of histocompatible primate-induced pluripotent stem cells for neural transplantation. Stem Cells 29, 1052–1063 (2011) CASPubMedArticle Laflamme, M. A. & Murry, C. E. Heart regeneration. Nature 473, 326–335 (2011) CASISIPubMedArticle Lalit, P. A., Hei, D. J., Raval, A. N. & Kamp, T. J. Induced pluripotent stem cells for post-myocardial infarction repair: remarkable opportunities and challenges. Circ. Res. 114, 1328–1345 (2014) CASPubMedArticle Shiba, Y., Hauch, K. D. & Laflamme, M. A. Cardiac applications for human pluripotent stem cells. Curr. Pharm. Des. 15, 2791–2806 (2009) CASPubMedArticle Bach, F. H., Bach, M. L. & Sondel, P. M. Differential function of major histocompatibility complex antigens in T-lymphocyte activation. Nature 259, 273–281 (1976) CASISIPubMedArticle Petersdorf, E. W. The major histocompatibility complex: a model for understanding graft-versus-host disease. Blood 122, 1863–1872 (2013) CASPubMedArticle Shiina, T. et al. Discovery of novel MHC-class I alleles and haplotypes in Filipino cynomolgus macaques (Macaca fascicularis) by pyrosequencing and Sanger sequencing: Mafa-class I polymorphism. 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A., Jankowska-Gan, E., Xu, Q. & Burlingham, W. J. Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1. J. Immunol. 179, 3443–3451 (2007) CASISIPubMedArticle Kwun, J. et al. Impact of leukocyte function-associated antigen-1 blockade on endogenous allospecific T cells to multiple minor histocompatibility antigen mismatched cardiac allograft. Transplantation 99, 2485–2493 (2015) CASPubMedArticle Vokaer, B. et al. Critical role of regulatory T cells in Th17-mediated minor antigen-disparate rejection. J. Immunol. 185, 3417–3425 (2010) CASPubMedArticle Kawamura, T. et al. Cardiomyocytes derived from MHC-homozygous induced pluripotent stem cells exhibit reduced allogeneic immunogenicity in MHC-matched non-human primates. Stem Cell Reports 6, 312–320 (2016) CASPubMedArticle Chong, J. J. et al. Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts. Nature 510, 273–277 (2014) CASISIPubMedArticle Robinson, J., Halliwell, J. A., McWilliam, H., Lopez, R. & Marsh, S. G. IPD--the immuno polymorphism database. Nucleic Acids Res. 41, D1234–D1240 (2013) CASISIPubMedArticle Okita, K. et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells 31, 458–466 (2013) CASISIPubMedArticle Ohkura, M. et al. Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7, e51286 (2012) CASPubMedArticle Bigaud, M., Maurer, C., Vedrine, C., Puissant, B. & Blancher, A. A simple method to optimize peripheral blood mononuclear cell preparation from cynomolgus monkeys and improve mixed lymphocyte reactions. J. Pharmacol. Toxicol. Methods 50, 153–159 (2004) CASPubMedArticle Download references Author information References• Author information• Extended data figures and tables• Supplementary information Author footnotes These authors contributed equally to this work. Yuji Shiba & Toshihito Gomibuchi Affiliations Institute for Biomedical Sciences, Shinshu University, Matsumoto 390-8621, Japan Yuji Shiba Department of Cardiovascular Medicine, Shinshu University School of Medicine, Matsumoto 390-8621, Japan Yuji Shiba & Uichi Ikeda Department of Cardiovascular Surgery, Shinshu University School of Medicine, Matsumoto 390-8621, Japan Toshihito Gomibuchi, Tatsuichiro Seto, Yuko Wada, Hajime Ichimura, Yuki Tanaka, Tatsuki Ogasawara & Kenji Okada Department of Pediatrics, Shinshu University School of Medicine, Matsumoto 390-8621, Japan Naoko Shiba Ina Research Inc., Ina 399-4501, Japan Kengo Sakamoto & Daisuke Ido Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Japan Takashi Shiina Brain Science Institute, Saitama University, Saitama 338-8570, Japan Masamichi Ohkura & Junichi Nakai Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan Masamichi Ohkura & Junichi Nakai Chromosome Engineering Research Center, Tottori University, Yonago 683-8503, Japan Narumi Uno, Yasuhiro Kazuki & Mitsuo Oshimura Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan Itsunari Minami Contributions Y.S. designed the study. Y.S., T.G., T.Se., Y.W., H.I., Y.T., K.S. and D.I. performed all animal procedures. T.O., N.S. and Y.S. performed histological analysis. K.S. and D.I. analysed Holter ECGs. N.U., Y.K., and M.Os. performed karyotype analysis of iPSCs. T.Sh. analysed RNA sequences of cynomolgus MHC. K.O. and U.I. analysed all other data and provided administrative assistance. M.Oh. and J.N. generated the G-CaMP7.09 plasmid. In vitro fluorescent imaging studies were performed by I.M. The manuscript was written by Y.S., T.Sh., M.Oh., I.M. and N.U. Competing financial interests K.S. and D.I. are employees of Ina Research, where all animal procedures in this study were performed. The remaining authors have no competing interests to declare. Corresponding author Correspondence to: Yuji Shiba Reviewer Information Nature thanks T. Braun, K. Fukuda, T. Kamp and the other anonymous reviewer(s) for their contribution to the peer review of this work. Extended data figures and tables References• Author information• Extended data figures and tables• Supplementary information Extended Data Figures Extended Data Figure 1: Characteristics of the HT4 haplotype. (649 KB) a, b, Basic structure of MHC in HT4 haplotypes. One of the cynomolgus monkeys (DrpZ5-32B-C) is strictly a ‘homozygote’ that has the A-Hp7.2 and B-Hp2 haplotypes in the Mafa-class I region and the #7 haplotype in the Mafa-class II region on both chromosomes (tentatively named ‘HT4’). c, In vitro mixed lymphoid reactions (MLR) showed that when inactivated lymphocytes from a HT4-heterozygous monkey were cocultured with active lymphocytes from a HT4-homozygous monkey, proliferation was inhibited to the level of control (only inactivated cells) or autologous (inactivated and active cells from same animal). ‘MHC mismatched’ indicates two groups of lymphocytes from two different animals with different MHC types. **P < 0.01 versus control. n = 5 per group. Extended Data Figure 2: Generation of iPSCs from a MHC homologous cynomolgus monkey. (774 KB) Donor iPSCs were established from skin fibroblasts by transfection of episomal vectors carrying OCT4, KLF4, SOX2 and L-MYC. a, iPSCs form typical ES-cell-like colonies. Scale bar, 50 μm. b–e, iPSCs express pluripotent markers as assessed by immunofluorescence. Scale bars, 100 μm. f, Gene expression of pluripotent markers in the iPSCs is identical to that in cynomolgus ES cells. g–i, When transplanted into immunodeficient mice, the iPSCs gave rise to teratomas manifesting all three germ layers: endoderm (intestinal epithelium), mesoderm (cartilage) and ectoderm (squamous cells). j, After expansion, the iPSCs showed normal karyotype (42, XY). Extended Data Figure 3: Characteristics of G-CaMP7.09. (217 KB) a, Schematic structure of G-CaMP7.09. Mutations are indicated with respect to G-CaMP7. RSET and M13 are tags that encode hexahistidine and a target peptide for Ca2+-bound CaM derived from myosin light chain kinase, respectively. The amino-acid numbers of EGFP and CaM are indicated in parentheses. The dynamic range of G-CaMP7.09 (Fmax/Fmin) was 19.3 ± 2.52 (n = 3) and the Kd for Ca2+ was 212 ± 6.9 nM (n = 3). b–h, In vitro fluorescence transients of G-CaMP7.09-expressing cardiomyocytes. Data are representative of three independent experiments. b, Spontaneous contraction. Scale bar, 2 s. c, The firing rate of G-CaMP signals was reduced by treatment with ryanodine, a ryanodine receptor blocker. Scale bar, 2 s. d, Addition of the L-type calcium-channel blocker, nifedipine, resulted in cessation of fluorescent transients. Scale bar, 6 s. e, Treatment with an activator of the ryanodine receptor, caffeine, induced fluorescent transients in the G-CaMP7.09-expressing iPSC-CMs. Scale bar, 6s. f, G-CaMP7.09 transients were sustained for a few minutes after spontaneous contraction and stopped by 40 mM BDM. Scale bar, 1 s. g, After cessation of spontaneous fluorescent transients, iPSC-CMs on Parafilm were stretched but no fluorescent transient was detected. Scale bar, 10 s. h, Treatment with caffeine induced G-CaMP7.09 transients again. Scale bar, 5 s. Extended Data Figure 4: Generation and purification of cynomolgus iPS cell-derived cardiomyocytes. (404 KB) a, Pilot experiments showed that cultivation of iPSC-CMs in glucose-free medium for 72 h significantly enhances cardiac purity, **P < 0.01 versus 0 h, n = 4 for each time point. Data are representative of three independent experiments. b, iPSC-CMs express the cardiac-specific marker cTnT. Scale bar, 50 μm. c, d, After multiple attempts to generate cardiomyocytes for transplantation, 2 × 109 cardiomyocytes (cTnT-positive 83.8 ± 1.0% as indicated by flow-cytometric analysis) were prepared. e, f, The cardiomyocytes were positive for GFP. g, RT–PCR analysis indicated that cTnT mRNA expression in iPSC-CMs was detectable, but lower than in the adult heart. Data are representative of three independent experiments. Extended Data Figure 5: Study protocol and design. (132 KB) a, A monolayer of cultured undifferentiated cynomolgus monkey iPSCs on a Matrigel (MG)-coated dish was treated with Matrigel. The culture medium was replaced with serum-free medium supplemented with Matrigel and activin A (AA) on day 0. On day 1 after activation, the medium was replaced with medium containing BMP4 and basic fibroblast growth factor (bFGF), and cells were cultured until day 5. On day 14, cardiomyocytes were selected by cultivation in glucose-free medium for 3 days. b, Fourteen days before transplantation, 10 female monkeys were subjected to ischaemia/reperfusion injury. Either 4 × 108 iPSC-CMs reconstituted in a prosurvival cocktail (PSC) or the PSC vehicle was injected on day 0. Cardiac μCT and UCG were performed to evaluate cardiac contractile function before and after transplantation. Additionally, BNP was measured. Spontaneous arrhythmias were monitored by Holter electrocardiogram (ECG) on days −1, 7, 14 and every other week thereafter. On day 84, all animals were euthanized, and the hearts were excised and subjected to intravital G-CaMP imaging, followed by histological analysis. Extended Data Figure 6: Immune response following transplantation of iPS cell-derived cardiomyocytes. (1,724 KB) a, b, iPSC-CMs were transplanted into MHC-mismatched infarcted hearts (n = 2). Animals were euthanized and the hearts were collected at 4 weeks post-transplantation. Only a small portion of grafts (GFP) showed a severe infiltration of inflammatory cells, such as CD3+ T lymphocytes. c–i, Immunohistochemical analysis of recipients of iPSC-CMs or PSC vehicle 84 days post-transplantation. The sections were stained with antibodies against CD45 (leukocytes), CD20 (B lymphocytes), CD3 (T lymphocytes) and GFP (graft). Scale bars in a–i, 200 μm. Extended Data Figure 7: Macroscopic and microscopic analysis of iPSC-CM recipients. (1,332 KB) a–h, All recipients of iPSC-CMs received full necropsy after euthanasia. Neither macroscopic (a–d) nor microscopic (e–h) analysis revealed any evidence of tumour formation at 12 weeks post cell transplantation. Scale bars in a–d and e–h: 10 mm and 200 μm, respectively.i–p, Additional immunohistochemical analysis of cynomolgus hearts. i, Immunohistochemistry for GFP (brown) counterstained with fast green. Scale bar, 1 mm. j, k, Picrosirius red staining of a section in close proximity to the visual field in a shows partial remuscularisation of the scar (shown in red) by grafted cardiomyocytes. l–n, Different sections (lower by 5 mm towards the apex) showing the corresponding 2 grafts from Fig. 1b. Scale bar, 200 μm. m, n, Magnified images of the grafts, scale bar, 50 μm. Note the more direct contact zone of grafted cardiomyocytes with host myocardium. o, p, Additional examples of grafted cardiomyocytes in the scar and the border zone. Scale bars, 200 μm. Extended Data Figure 8: Summary of histological, mechanical and electrophysiological consequences. (366 KB) a, Animal characteristics with histological, mechanical and calcium imaging results. b, Correlation between ejection fraction (EF) and scar area relative to left ventricular area (LV). c, Correlation between ejection fraction and graft area relative to left ventricle. d, Summary of sustained ventricular tachycardia (VT), including number of VTs, maximum duration, and maximum heart rate (HR), in the recipients of iPSC-CMs. Extended Data Figure 9: Additional electrical analysis of hearts transplanted with iPSC-CMs. (305 KB) a, b, Activation map obtained from G-CaMP7.09 transients showing the interval (in ms) between the R wave of ECG and the peak of the G-CaMP7.09 fluorescent signal. c–f, Examples of sustained and non-sustained VT in recipients of iPSC-CMs. Arrows indicate P wave during VT, suggesting atrioventricular dissociation. Scale bar, 1 s. Extended Data Figure 10: Time course of left ventricular size and BNP levels. (196 KB) a–d, Left ventricular size was analysed before transplantation (Pre-Tx), 4 weeks post-transplantation (4 w post-Tx) and 12 weeks post-transplantation (12 w post-Tx) by echocardiography (a, b) and μCT (c, d). LVEDD: left ventricular end-diastolic dimension, LVESD: left ventricular end-systolic dimension, LVEDV: left ventricular end-diastolic volume, LVESV: left ventricular end-systolic volume. n = 5 per group. #P < 0.05; ##P < 0.01. * P < 0.05; **P < 0.01 versus Pre-TX. e, BNP was measured on days 0 (14 days after myocardial infarction), 28, 56 and 84. No significant difference was detected between recipients of iPSC-CMs and recipients of PSC vehicle at any time point. *P < 0.05 versus day 0. Supplementary information References• Author information• Extended data figures and tables• Supplementary information Video Video 1: In vitro fluorescent imaging of G-CaMP7.09-expressing cynomolgus iPSC-CMs (10.37 MB, Download) Monolayer-cultured cardiomyocytes exhibit robust fluorescent flashes in synchrony with their contraction. Video 2: In vitro experiments with G-CaMP7.09-expressing iPSC-CMs by using nifedipine, ryanodine, and caffeine (15.49 MB, Download) Representative video of G-CaMP7.09 fluorescent transients and contraction of cardiomyocytes before and after treatment with the L-type calcium-channel blocker nifedipine, the ryanodine receptor blocker ryanodine, and the ryanodine receptor activator caffeine. Video 3: In vitro G-CaMP7.09 transients sustained after cessation of spontaneous contraction of iPSC-CMs by BDM. (6.01 MB, Download) Treatment with 40 mM BDM resulted in cessation of spontaneous contraction of iPSC-CMs, but G-CaMP7.09 fluorescent transients were sustained for a few minutes. Video 4: Myocardial ischemia/reperfusion model in cynomolgus monkey (19.84 MB, Download) The myocardial infarction model was produced by 3 h of ischemia followed by reperfusion using polyethylene tubing 2 weeks before transplantation. Video 5: Intravital imaging of G-CaMP7.09-expressing iPSC-CMs in cynomolgus heart (9.49 MB, Download) G-CaMP7.09⁺ iPSC-CMs were transplanted into infarcted cynomolgus hearts. The hearts were excised and mechanically arrested ex vivo by perfusion with BDM on a Langendorff apparatus at 12 weeks post-transplantation. All graft regions exhibited cyclic changes in fluorescent intensity that occurred synchronously in a 1:1 relationship with the host ECG when the heart beat spontaneously or was electrically paced at rates from 3 to 5 Hz. Note that some, but not all hearts could be paced up to 5 Hz. Video 6: Cardiac contractile function assessed by mCT at 4 weeks post-transplantation (3.34 MB, Download) Cardiac function was assessed by mCT pre- and post-transplantation. The first and second segments of the video show left ventricular contractions of the short axis at the base and apex, respectively. Note that while contraction at the base is similar, that at the apex in iPSC-CM recipients looks better than that in PSC-vehicle recipients. The last segment represents a long-axis view of the left ventricle.

Nature Biomedical Engineering Page Liked · 7 hrs · Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Published in Nature. rdcu.be/lGxF

regenerates primate hearts

Thursday, October 6, 2016

Salt, pepper and lemon are not just great in salad – they can treat a wide range of diseases and conditions naturally and without side-effects. Here’s what they can help you with:

Salt, pepper and lemon are not just great in salad – they can treat a wide range of diseases and conditions naturally and without side-effects. Here’s what they can help you with: Sore throat Mix a tablespoon of lemon juice, half a teaspoon of black pepper and a teaspoon of salt in a glass of warm water and gargle the mixture a couple of times a day to relieve your sore throat and prevent coughs. Stuffy nose In order to relieve your stuffed nose, you need to sneeze, and you can do that right away if you sniff a mixture of black pepper, cinnamon, cumin and cardamom. Sniff the mixture slowly and you’ll relieved your stuffy nose instantly. Remove gallstones Gallstones will obstruct your digestive system and can be very painful. They are hardened deposits of digestive fluids that form in your gallbladder and needs to be resolved as soon as possible. In order to do that, you need to take a mixture of 3 parts olive oil and 1 part of lemon and black pepper. Weight loss The polyphenols in lemons can prevent weight gain and improve your insulin sensitivity which will result in better fat burn. To accelerate your metabolism and burn more fat, you should drink a mixture of ¼ of a teaspoon of ground black pepper, 2 tablespoons of lemon juice and a tablespoon of organic honey mixed in a glass of warm water. Drinking the mixture regularly will provide incredible results! Nausea Mix a tablespoon of freshly squeezed lemon juice and a teaspoon of black pepper in a glass of warm water and drink the mixture to relieve nausea and soothe your stomach. Asthma attack Boil some water in a pot, then add 10 grains of pepper, 2 clove buds and 15 basil leaves and simmer the mixture for 15 minutes. Afterwards, strain the mixture in a cup, then add 2 tablespoons of honey once the drink has cooled down. You can keep the asthma remedy in the fridge for up to 2 weeks. Toothache A mixture of clove oil and ground pepper can successfully relieve toothache when applied on the aching spot. Cold and flu Squeeze half a lemon into a cup of boiled water, then let it steep for 10 minutes. Remove the lemon in the end and drink the cold and flu remedy while it’s still warm. Add some honey to taste. Nosebleeds In order to stop a nosebleed immediately, apply some lemon juice on a cotton ball and put it in your nostril – just make sure you tilt your head slightly forward in order to stop the blood from dripping into your throat and prevent nausea. source : http://www.healthylifevision.com

This Oil Destroys 93% of Colon Cancer Cells in 2 Days Written By Admin on Monday, July 18, 2016 | 4:53:00 AM

Coconut Oil Destroys 93% of Colon Cancer Cells in 2 Days? After all these years, coconut oil is getting the positive scientific attention it deserves. There’s a reason cultures around the world have been using it for thousands of years. The benefits of coconut oil have filled entire books. There are very good reasons for that. Full of healthy saturated fats, coconut oil is a great source of fatty acids, which are commonly found in fish and seeds. In fact, it is one of the richest sources of saturated fat known to man, with almost 90% of the fatty acids in it being saturated . Coconut oil has previously been shown to help decrease the harmful side effects of chemotherapy, and improve the quality of life for cancer patients. “According to a study published in the journal Cancer Research, the lauric acid in coconut oil has anti-cancer properties. Lauric acid constitutes about 50% of coconut oil, and researchers at the University of Adelaide discovered that it completely exterminated more than 90% of colon cancer cells after just two days of treatment in a colon cancer cell line in vitro. Researchers also cited other studies that support their findings.” Nearly 50% of coconut oil’s fatty acids contain lauric acid, which has been praised as able to kill any harmful pathogens that enter the human body. In this study, lauric acid actually ‘poisoned’ cancer cells and unleashed tremendous oxidative stress, while at the same time reducing glutathione levels. This experiment was performed “in vitro”(it was performed on cells outside of their normal biological system), which may cause some to discount the findings. Performing more research costs money — lots of money — which is hard for researchers to come by without a pharmaceutical company backing them.